Exo-Resistant Random Primer

Product name:Exo-Resistant Random Primer

Descriptions: MCLAB's Exo-Resistant Random Primer is a mixture of single-stranded random oligonucleotides. It can be used in many applications such as highly efficient random priming of various DNA synthesis reactions. The primer in this prodcut has two 3’-terminal phosphorothioate (PTO) modifications that are resistant to the 3'=>5' exonuclease activity of proofreading DNA polymerases (1), like Klenow Fragment and phi29 DNA Polymerase. It also has 5’- and 3’-hydroxyl ends. The product is supplied as a ready-to-use, 20X concentrated aqueous solution.

Applications:
Strand displacement amplification of genomic DNA (2), plasmids and phage DNA (3).
DNA labeling by random priming (4-6).