Malonate Broth

Product Name:Malonate Broth

Product Description:Malonate Broth is prepared according to the formula described by Leifson.1 Malonate Broth is a liquid medium containing ammonium sulfate as the only source of nitrogen, and malonate as the only source of carbon. During Leifson’s experiments the Enterobacter group utilized malonate, producing an alkaline reaction and changing the color of the medium from green to blue. The Escherichia group did not utilize malonate (medium remained green) and failed to grow.
Malonate Broth is further described for differentiating Enterobacteriaceae in food and dairy products.2-4 In certain cases the medium referenced is the Edwards and Ewing formulation that contains yeast extract and dextrose. Adding yeast extract and dextrose permits growth of organisms that would otherwise fail in Malonate Broth.

Intended Use:Malonate Broth is used for the differentiation of Enterobacter spp. and Escherichia spp. on the basis of malonate utilization.