NlaIV restriction enzymes

Product name:NlaIV restriction enzymes

Restriction Site Sequence:
...GGNNCC...
...CCNNGG...

Restriction Site Sequence After Cut:
...GGN NCC...
...CCN NGG...

Restriction enzyme reaction conditions:37oC

Compatible ends (at this website):BspLI, PspN4I

Heat Denaturation:NlaIV can be denatured by heating at 65癈 for 20 minutes

Genomic source:Neisseria lactamica is the genomic source for NlaIV

Product Description:A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences known as restriction sites. Such enzymes, found in bacteria and archaea, are thought to have evolved to provide a defense mechanism against invading viruses. Inside a bacterial host, the restriction enzymes selectively cut up foreign DNA in a process called restriction; host DNA is methylated by a modification enzyme (a methylase) to protect it from the restriction enzyme’s activity. Collectively, these two processes form the restriction modification system. To cut the DNA, a restriction enzyme makes two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.