Rnase H minus M-MuLV Reverse Transcriptase

Product name:Rnase H minus M-MuLV Reverse Transcriptase

Origin:recombinant from E. coli from Moloney Murine Leukemia Virus

Descriptions:M-MuLV RT, RNase minus (recombinant modified form of the Reverse Transcriptase from the Moloney Murine Leukemia Virus with inactivated RNase H activity) is an RNA-dependent DNA polymerase. The enzyme is a product of a pol gene of M-MuLV and consists of a single subunit with a molecular weight of 69 kDa. M-MuLV RT has no intrinsic RNase H activity. This enzyme does not demonstrate any degradation of the RNA molecule during first strand cDNA synthesis and gives substantially greater yields of first strand cDNA, than obtained with any other reverse transcriptase.

Applications:First strand DNA synthesis for cloning and hybridization, filling-in and labelling the 3’-terminy of DNA with 5’-protruding ends, DNA sequencing, and RT-PCR.