SYBR green

Product name:SYBR green

CAS:163795-75-3

Molecular formula:C32H37N4S+
 
Product description:SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. SYBR Green I binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). The stain preferentially binds to double-stranded DNA, but will stain single-stranded DNA with lower performance. SYBR green can also stain RNA with a lower performance than DNA.SYBR green finds usage in several areas of biochemistry and molecular biology. It is used as a dye for the quantification of double stranded DNA in some methods of real time PCR. It is also used to visualise DNA in gel electrophoresis. Higher concentrations of SYBR green can be used to stain agarose gels in order to visualise the DNA present. In addition to labelling pure nucleic acids SYBR green can also be used for labelling of DNA within cells for flow cytometry and fluorescence microscopy. In these cases RNase treatment may be required to reduce background from RNA in the cells.