Thermosensitive Alkaline Phosphatase

Product name:Thermosensitive Alkaline Phosphatase

Descriptions:TSAP thermosensitive alkaline phosphatase is a robust phosphatase that can efficiently dephosphorylate all 5 termini from DNA fragments in 15 minutes at 37°C. TSAP is active in Promega restriction enzyme buffers as well as GoTaq PCR Buffers. This allows TSAP to be used in simultaneous restriction digest/dephosphorylation reactions without the need for buffer exchange or serial reactions. TSAP is also irreversibly inactivated by incubating at 74°C for 15 minutes. Therefore, a DNA cleanup step is not required before proceeding to a ligation reaction. Here we examined whether TSAP can be used directly in reactions with NEB’s common restriction buffers.