m-FC Broth Base

Product Name:m-FC Broth Base

Principles of the Procedure:Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue provide nitrogen, carbon, and minerals in m-FC Broth. Yeast Extract is a source of vitamins and trace elements. Sodium Chloride maintains the osmotic balance. Lactose serves as a carbohydrate source. Bile Salts inhibit growth of Gram-positive bacteria. The differential indicator system combines Aniline Blue and Rosolic Acid, which is added as a supplement.

Product Description:Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in several procedures.2,3 The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.

Intended Use:m-FC Broth is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration.