pTG19-T Vector

Product name:pTG19-T Vector

Descriptions:pTG19-T vector is designed to directly clone PCR products with a overhang dA residue at 3’ end produced by Taq DNA polymerase, or other polymerases. This vector is derived from pTG19R, and it has all the feature of pTZ19 except the sequence of multiple clone site.

Specialized applications of the pTG19-T vector:
1.Cloning PCR products.
2.Blue/white screening for recombinants.
3.Confirming the white clone with unique restriction endonuclease BamHI.
4.Sequencing the insert with universal primer M13 forward primer, M13 reverse primer or T7 promoter primer.
5.Using for construction of unidirectional nested deletions.
6.In vitro transcription from T7 promoter.