pUC19 DNA/MspI (HpaII) Marker, 23

Product Name ：pUC19 DNA/MspI (HpaII) Marker, 23

Package ： 5x50ug 
 
Description ： Quality Control
Tested in appropriate gel electrophoresis. The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.
 
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
 
Storage and Loading Buffer (for ready-to-use markers)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.
 
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Conventional phage and plasmid DNA markers are recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels.
Phage or plasmid DNA is digested to completion with the appropriate Fermentas  restriction enzyme, then purified and dissolved in storage buffer. DNA fragments contain various ends (blunt or sticky) depending on the restriction enzyme used for marker preparation.
pUC Mix, PhiX174 DNA/BsuRI, pBR322 DNA/AluI and pUC19 DNA/MspI markers are also available in ready-to-use versions - premixed with 6X DNA Loading Dye for direct loading onto agarose and polyacrylamide gels. All markers are supplied with 6X DNA Loading Dye.
 
Features
1.Recommended for sizing and approximate quantification of small DNA fragments;
2.Produce sharp bands;
3.Conventional versions (supplied in storage (TE) buffer) can be labeled radioactively with T4 Polynucleotide