CASPASE-3 elisa kit

Product name : CASPASE-3 elisa kit

Synonyms : Human cysteinyl aspartate specific proteinases 3,Caspase-3/CPP32 ELISA Ki

Product description :
This immunoassay kit allows for the specific measurement of Human Caspase 3,Casp-3 concentrations in cell culture supernates, tissue homogenates, serum, and plasma. Caspases are a family of calcium-dependent cysteine proteases, which play essential roles in apoptosis (programmed cell death), necrosis and inflammation.
Caspases are essential in cells for apoptosis, one of the main types of programmed cell death in development and most other stages of adult life, and have been termed "executioner" proteins for their roles in the cell. Some caspases are also required in the immune system for the maturation of cytokines.
Caspase-3, also known as CPP-32, Yama or Apopain, is an intracellular cysteine protease that exists as a proenzyme, becoming activated during the cascade of events associated with apoptosis. Caspase-3 cleaves a variety of cellular molecules that contain the amino acid motif DEVD such as
poly ADP-ribose polymerase (PARP), the 70 kD protein of the U1-ribonucleoprotein and a subunit of the DNA dependent protein kinase. The presence of caspase-3 in cells of different lineages suggests that caspase-3 is a key enzyme required for the execution of apoptosis.
Caspase 3 is a caspase protein. It interacts with caspase 8. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.