Hot start Taq DNA polymerase(500U)

[Products Name]  Hot start Taq DNA polymerase(500U)

FOR RESEARCH USE ONLY

Description:
Same outstanding polymerase as eDNA standard Taq
Polymerase activity inhibited by Taq antibody until initial denaturation temperature is reached
Ultrapure grade enzyme – all inhibitor/DNA contamination removed by affinity chromatography

 Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5?->3? polymerase activity and a double-strand specific 5?->3? exonuclease activity.

 Isolated from an E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus

 Performs well over a wide range of templates

 Incorporates dUTP, dITP and fluorescently-labeled nucleotides

 Supplied with 10X Reaction Buffer and 25mM MgCl2

 Applications:

 Routine PCR

 Real-Time PCR

 Primer extension

 Single-colony PCR

 Nick translation

 A-tailing of blunt dsDNA fragments for T-vector cloning

 End-labeling blund dsDNA fragments

 Enzyme Properties

 3? to 5? exonuclease activity: No

 5? to 3? exonuclease activity: Yes

 Strand Displacement: No

 Heat Inactivation: No

 Theoretical Molecular Weight: 94,000 daltons

 Error Rate: 285 x10-6 bases

 Reaction Conditions:
1X Standard Taq Reaction Buffer:
 
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP in 30 minutes at 75°C.

Unit Assay Conditions:
1X Standard Taq Reaction Buffer, 200 ?M dNTPs including and 200 ?g/ml ssDNA template.2

Quality Analysis Assays

Absense of 3’to 5’ exonuclease activity
Absence of endonuclease activity
Absence of DNA contamination
Standard PCR positive and negative controls

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
1mM PMSF
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% Igepal CA 630

Storage Temperature:
-20°C

Patent Info
Use of this product for basic PCR is outside of any valid US patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees

1. U.J. Desai and P.K. Pfaffle, Biotechniques 19, 780 (1995)
2. H. Tveit and T. Kristensen, Analytical Biochemistry 289, 96 (2001)